The activity of enzymes can be modified by homeopathic dilutions of their effectors.
Introduction: The fungal and bacterial materials are very useful for testing the
influence of low and very low doses of low molecular phenolic effectors on enzymatic
system of phenoloxidases when they are incubated together in the reaction space.
Aim: Searching for the model useful biological systems to study the action of diluted
low molecular substances on living organisms, which is based on common physical
and biochemical analytical procedures. Methods: The fungal and actinomycetal
bacterial materials from laboratory cultures as a source of common phenoloxidases,
laccase, peroxidase and O-demethylase as well as the pure plant peroxidase were
used in experiments described earlier [1-5]. Subsequent dilutions of low molecular
phenolic metabolites, appropriate for studied enzymatic systems, prepared in 75%
ethanol in the proportion of 1:100 (centesimal) and dynamized by shaking in
accordance with homeopathic procedures were prepared in our laboratory. During
experiments with bacterial and fungal materials and a pure plant peroxidase, which
were incubated together with subsequent dilutions of proper phenolic effector,
different analytic methods were used including a gel (PAGE)  and capillary
(MEKCE) electrophoresis , spectral and colorimetric methods [1,2,3] as well as the
electron microscopy . Results: In the light of presented data [1-5], the incubation of
biological material with diluted phenolic effectors induces various effects on tested
enzyme activity. It changed in sinusoidal manner with an gradual growth of dilution
rate of tested effectors, which was distinctly visible on the diagram when the number
of dilutions was localized on abscissa and biological activity on the ordinate.
Exemplary results of the chosen experiments will be presented. For tested enzymes:
laccase, peroxidase and O-demethylase, the distance between maximal points of
enzymatic activity, shown on a sine curve, repeats more often every 10 subsequent
centesimal dilutions. Along with the extension of incubation period the displacement
of maximal and minimal points on curve were noticed, which revealed a dynamic
aspect of studied phenomenon. Conclusions: Fungal and bacterial cells seem to be
a very convenient material for studying the action of diluted metabolites on
enzymatic systems because their popular presence in environment. Results of all
experiments confirmed the same nature of the mentioned observations. Because
other authors had similar conclusion concerning human [6,7] and plant materials
[8,9], the described relations seem to be common in natural world. It could also be
stressed that the therapeutic effect of homeopathic remedies could be based on the
mechanism described above and it is highly probable that it leads to a normalisation
of disturbed enzyme systems in the living organisms.
Research papers concerning agrohomeopathy, homeopathy (if relevant), potentised BD remedies and so forth.