Phytopathological and nutraceutical evaluation of cauliflower plants treated with high dilutions of arsenic trioxide.
Trebbi G, Dinelli G, Marotti I, Bregola V, Brizzi M, Betti L.
Introduction: This research aimed at verifying the effects of highly diluted (HD)
treatments on cauliflower (Brassica oleracea L.) plants both healthy and inoculated
by the fungus Alternaria brassicicola, causing the dark leaf spot disease. In vitro
spore germination assays (A), growth chamber experiments (B) and field trials (C)
Material and Methods: (A): spore suspensions were prepared in HD treatments and
their inhibiting effect on germination was recorded microscopically after incubation at
25°C for 5 h. (B): the same treatments were tested in plants artificially inoculated
with the fungus. The infection level on leaves was blindly evaluated by a previously
defined infection scale. (C): the field was divided into plots according to a complete
randomized block design. In the first trial (i), plants were artificially inoculated and
weekly treated; the infection level was evaluated on cauliflower heads. The second
trial (ii) was performed on the same field with the aim to induce a natural infection,
mediated by infected crop residues. Measurement endpoints concerned the
evaluation of some physiological parameters along with the glucosinolate content on
Results: (A): arsenic trioxide (As 35x and 35x diluted 1:5000) and Cuprum 5x
induced highly significant inhibition of germination rate (-60%) vs. control. (B): As 35x
and Cu 3 g/l induced a significant decrease of mean infection level (-50%). (C): in (i),
a significant reduction of disease symptoms on heads was recorded for As 35x and
Cu 3 g/l (-45%). In (ii) natural fungal infection did not occur due to dry weather
conditions; physiological and nutraceutical analyses of healthy heads demonstrated
that As 35x induced a significant increase of both head size and glucosinolate
Discussion: Some evidences on the efficacy of arsenic, at different decimal and
centesimal HD, in fungal and viral disease control were previously reported . In the
present study the efficacy of HD arsenic in dark leaf spot control in field has been
shown for the first time: since fungal inoculation was performed on the leaves before
flowering, we can hypothesize that this treatment induced an increase of plant
resistance to fungal infection.
Research papers concerning agrohomeopathy, homeopathy (if relevant), potentised BD remedies and so forth.